Chapter 2 Find Doublet using Scrublet

2.1 description

Scoring and identify doublet using scrublet per samples

2.2 input data

filtered count matrix by cellRanger per samples

2.3 process

• running scrublet per samples

• N2-DoubletIdentify-Scurblet-Process.py -i Pre-A -r 0.02

  • expected doublet ratio is calculated by ~ 0.8% per 1k cell
  • other parameters by default

2.4 output

• doublet scoring and identificaiton

  • data/scrublet/*DoubletScores.csv
  • data/scrublet/*PredictedDoublets.csv

• validation plots

  • data/scrublet/pdf