LSM 980 Guide
1
Start Page
1.1
About This Guide
1.2
About the Tumor Cartography Core
1.2.1
Address
1.2.2
Contacts
1.3
Billing Information
1.4
Troubleshooting
2
General Setup Procedure
2.1
Turning On
2.2
Mount Your Sample
2.3
Locate Your Sample
2.4
Use the AI Sample Finder
2.5
Switch to the Desired Objective
2.6
Adjust the Stage to an Acceptable Height
2.7
Start Image Acquisition
3
Single Image Acquisition
3.1
Airyscan SR and Multiplex Modes
3.1.1
Set Up Tracks and Acquisition Scheme
3.1.2
Ensure You Can See Something & Get in Focus
3.1.3
Toggle through the Tracks and Optimize View Settings
3.1.4
Airyscan Detector Alignment
3.1.5
Capture an Image and the Associated Data
3.1.6
Perform Airyscan Processing to Acquire a Final Flat Image
3.2
Confocal Mode
3.2.1
Set Up Tracks and Acquisition Scheme
3.2.2
Adjust Your Acquisition Scheme
3.2.3
Ensure You Can See Something & Get in Focus
3.2.4
Toggle through the Tracks and Optimize View Settings
3.2.5
Capture an Image and the Associated Data
3.3
Widefield Mode
3.3.1
Set Up Tracks and Acquisition Scheme
3.3.2
Ensure You Can See Something & Get in Focus
3.3.3
Toggle through the Tracks and Optimize View Settings the laser power and gain settings for each track.
3.3.4
Capture an Image and the Associated Data
4
Imaging Considerations
4.1
When to Use SR Mode
4.2
Capturing Information in Z for Increased Resolution
4.3
Oversampling in XY For Increased Resolution
4.4
When to Use Multiplex Mode
4.5
When to Use Confocal Mode
4.6
Considering Excitation Bleedthrough
4.7
Line Scanning vs. Frame Scanning
4.8
Widefield Mode: A Completely Different Optical System
5
Tiling
6
Z-Stack
7
Time Series
8
General Shutdown Procedure
9
Troubleshooting
9.1
I Cannot See Anything
9.2
My Images All Have Low Signal in the Same Spot
10
Microscopy Concepts To Know
10.1
Blocks
10.2
Equations
10.3
Theorems and proofs
10.4
Callout blocks
11
References
11.1
Footnotes and citations
11.2
Footnotes
11.3
Citations
11.4
Sharing your book
11.5
Publishing
11.6
404 pages
11.7
Metadata for sharing
Published with bookdown
A Guide to the Zeiss LSM 980 with Airyscan 2
Chapter 5
Tiling