2.2 Early Microscopists and their Microscopes

Robert Hooke and Antoine van Leeuwenhoek were two early microscopists whose works with the microscope profoundly contributed to modern Biology.

2.2.1 Robert Hooke (1653 - 1703)

(from left to right) Robert's Compound Microscope, his Published Work, and some of his Sketches.

Figure 2.4: (from left to right) Robert’s Compound Microscope, his Published Work, and some of his Sketches.

He was a Dutch scientist whose work with the compound microscope led to the discovery of Cell Biology.

2.2.2 Antoine van Leeuwenhoek (1632 - 1723)

A Painting of Antoine and the Microscope that he Used.

Figure 2.5: A Painting of Antoine and the Microscope that he Used.

Leeuwenhoek was a Dutch draper (i.e., cloth importer) able to identify the following organisms via his simple microscope. He carried a magnifying glass to inspect the quality of cloths (as part of his job):

  1. Protists
  2. Bacteria
  3. Sperm
  4. Vacuoles (i.e., an organelle)

Leeuwenhoek had no formal training in Science - however, he was still able to make a microscope via his passion (albeit a little small - see above graphic).

2.2.3 Comparing Hooke’s and Leeuwenhoek’s Microscopes

Chromatic and Spherical Aberrations of a Wind Vane through a Telescope

Figure 2.6: Chromatic and Spherical Aberrations of a Wind Vane through a Telescope

The following table notes some differences between Hooke’s and Leeuwenhoek’s microscopes. The above image also shows how an object may appear through a lens when it is affected by chromatic and spherical aberrations.

Scientist Difference
Hooke Had chromatic and spherical aberrations
Leeuwenhoek Superior to Hooke’s compound microscope

An abberation (in the context of microscopy) is an imperfection in the final image seen by the viewer.

A spherical abberation happens when the light rays through a convex lens do not all converge to a single focal point. Consequently, the final image viewed is blurry (i.e., like a low-quality camera trying to zoom in - see the picture of the wind vane above).

Demonstration of Spherical Abberation

Figure 2.7: Demonstration of Spherical Abberation

A chromatic abberation is an imperfection that happens when the outline of an object becomes colored (see above image of wind vane again).