This book is a public facing adaption of the ‘general knowledge’ book created for the McConkey Lab. The ‘general knowledge’ book sought to serve as a central, standardized, and searchable repository for protocols, tailored to the needs of members in the McConkey lab. This book provide excerpts from that book that may be useful to more than those just within the McConkey lab. While I’ve made attempts to remove or change overly McConkey-specific portions of these protocols, you may need to adapt them to your own needs.